Fig. 2. Localization of laminin A. Wild-type animals were stained using laminin A antibodies. (A) The laminin A protein is detected in the late gastrula between the rows of intestinal (i) and pharyngeal (p) precursor cells, the flanking myoblast cells (m), and the epidermoblast cells (e). Mid-plane optical section. (B) During embryo elongation, laminin A is strongly detected between muscle and epidermal cells. Shown is staining associated with the two dorsal muscle quadrants. Dorsal lateral optical section. (C) In two- and threefold embryos, intense staining for laminin A is observed at the basement membranes associated with the pharynx and intestine. Staining is also detected at the nerve ring (nr). (D) In larvae and adults, staining is detected at the spermatheca (s) where the protein localizes between individual endothelial cells. (E) In larvae and adults, staining is weak at pharyngeal and intestinal basement membranes but is stronger at the nerve ring. (F) Laminin A is also detected in the basement membranes associated with the excretory canal (c). Anterior is towards the left, dorsal towards the top. Scale bars: 10 µm.