Fig. 4. Purkinje cell-specific inactivation of Rb and p107 at P30. Purkinje cells
are enlarged, show bizarre nuclei, and are occasionally displaced into the
molecular layer (D,G) when compared with control (A). Calbindin staining shows
a largely intact dendritic arborization in the molecular layer (E,H), similar
to wild-type cerebella (B). The cell cycle inhibitor p27 is accumulated in the
nucleus of most Rb- or Rb/p107-deficient Purkinje cells (F,I), when compared
with wild-type cells (C). Controls, n=2;
L7cre;RbLoxP/LoxP, n=3; L7cre;Rb
LoxP/LoxP;p107-/-, n=3. Scale
bar: 100 µm in A,B,D,E,G,H; 200 µm in C,F,I.