Fig. 6. Lack of p53 does not rescue cerebellar neurons deficient for Rb and p107. TUNEL staining of control (A), En2cre; Rb^LoxP/LoxP; p107^+/- (B) and En2cre; Rb^LoxP/LoxP; p107^+/-; p53^-/- (C) cerebella at postnatal day 15 shows no difference in the extent of apoptosis upon loss of p53. The cell layers in the double mutant (B,C) are indicated (EGL, external granular layer; PCL, Purkinje cell layer; IGL internal granular layer). Broken red lines indicate the inner border of the presumed IGL, which would normally form the cerebellar white matter. The cerebellum of a wild-type littermate is shown on the left as a control (A). Scale bar: 50 µm.