Fig. 2. Expression pattern of ceh-2 in the anterior pharynx, determined by anti-peptide antibody staining (A,C) and gfp reporter constructs (D-F). Animals are oriented with anterior towards the left; cells are labeled. (A,B,E) Right subdorsal views to show all cell types; (D) dorsal view; (F) ventral view showing both pairs of M3 and NSM neurons. (A,C) An antibody against a CEH-2 peptide stains 14 nuclei in the anterior pharynx of larvae (A) and five or six cells in gastrulating embryos (C). (B) Pharynx muscle corresponding to A, stained with 3NB12 mouse monoclonal antibody (Miller et al., 1983) against a pharyngeal myosin. (D-F) ceh-2::gfp under the control of 1.6 kb promoter sequence (pTRB 201) in wild-type (D,E) and ceh-2(ch4) animals (F) is expressed in the embryonic pharynx primordium (D) and neurons of the pharynx (E,F). ceh-2::gfp expression levels and cell morphology in ceh-2(ch4) mutants are unchanged (F).