Fig. 3. Neurons can be phenotyped by morphology and/or HuC-GFP expression. (A,B) Dorsal view of four neurons at 36 hpf in the live embryo. Scale bars: 20 µm. (A) Examining the cells by means of dextran visualization alone does not provide conclusive evidence of cell phenotype. (B) Coincidence of dextran-labelled cells with HuC-GFP confirms the cells' phenotype as neuronal. (C) Dorsal view of four neurons at 36 hpf in another live embryo. These four cells can be phenotyped by morphology alone by virtue of their axons. Scale bar: 20 µm. (D) Confocal projection of a clone in a fixed embryo at 48 hpf. Four cells with a neuronal morphology are seen on the right-hand side of the embryonic midline and two cells with a neuronal morphology on the left-hand side. Transverse view with dorsal to the top. Scale bar: 50 µm. (E) Confocal projection of the same specimen as documented in D shows coincident expression of all labelled cells with HuC-GFP. Here we phenotyped cells by morphology and transgene expression. Scale bar: 50 µm.