Fig. 2. Reintroduction of the missing cell type restores stripes in fms
and nac. (A,B) Transplantation of ß-Actin GFP expressing
golden mutant cells into fms hosts. (A) Normally pigmented,
donor-derived fms mutant melanophores form a stripe pattern if they
are in proximity of xanthophores. Melanophores further away from the
xanthophore clone form the typical melanophore clusters seen in fms
mutants. (B) UV-illumination shows GFP+, wild-type xanthophores forming
stripes with fms mutant melanophores. The xanthophores form a
coherent domain; however, several xanthophores have invaded the melanophore
stripe (arrows). Some of the GFP signal within the melanophore stripe is due
to transplanted cells underlying the melanophore stripe. (C,D) Transplantation
of bpeGFP cells into nac hosts. (C) Melanophores form stripes of
relatively normal position and size. (D) Fluorescence image of the same
sample. Host-derived xanthophores (arrows) display pteridine autofluorescence
around their nuclei. GFP+ xanthophores (arrowheads) show fluorescence in the
entire cell. In the proximity of melanophores, both donor- and host-derived
xanthophores organise into stripes, whereas outside the melanophore clone,
xanthophores form the nac mutant pattern.