Fig. 4. Ontogeny of the pigment pattern in obe (A,B) and leo (C,D) during larva to adult transition. (A) In obe, the initial distribution of melanophores at the onset of stripe formation (9 mm/22 d) is similar to the wild-type situation. Later (11 mm/24 d), melanophores fail to aggregate and remain evenly scattered. After the onset of xanthophore differentiation (11.5 mm/26 d), melanophores fail to be cleared from within the xanthophore stripe (13.5 mm/29 d, arrowhead). (B) The early (13.5 mm/29 d) distribution of xanthophores in obe is similar to wild type. Later (15 mm/31 d), differentiating xanthophores appear in between the scattered melanophores, giving rise to a mingled pattern. (C) leo mutants show a similar early (10 mm/23 d) melanophore pattern as in wild type. Later (11.5 mm/26 d), melanophores fail to cluster and converge towards the horizontal myoseptum and remain within the xanthophore domain (13.5 mm/29 d, arrowhead). (D) The early positioning of xanthophores in leo is similar to wild type, but later (14 mm/30 d) xanthophores start to differentiate within the melanophore domains, with some xanthophores encircling several melanophores (arrowhead).