Fig. 5. PVR function in border cell migration. (A,B) Wild-type expression of PVF1 (green) in the oocyte and nurse cell cytoplasm and PVR (red) in follicle cells at stage 8 (A) and stage 9 (B). PVR is expressed in the border cells (arrows). (C,D) PVR expression (red) in the outer follicle cells of an egg chamber with several Pvr^c2195 mosaic clones. Loss of GFP (green in D) marks cells homozygous mutant for Pvr (broken lines surround the mutant clones). (E,F) Egg chambers with border cells (arrows) mutant for Pvr. GFP (green) positively marks the homozygous mutant cells and filamentous actin (red) outlines all cells. (E) Egg chamber in which the mutant border cell cluster (arrow) exhibited delayed migration. (F) Egg chamber in which the mutant border cell cluster (arrow) migrated to the oocyte. (G) Quantitation of the Pvr mutant migration defect compared with the Pvf1 migration defect. Quantitation of border cell migration shown as the proportion of egg chambers in which the border cells migrated 0-25% (yellow), 26-50% (blue), 51-75% (red) and 76-100% (black) of the normal distance. Anterior is towards the left in all panels.