Fig. 1. Correlation of Wnt5a and Wnt11 expression with myogenic differentiation. The expression of Wnt5a (A-C,J-M), MyoD (D-F,N) and Wnt11 (G-I,N) in stage 22 (A,D,G), 25 (B,E,H) and 27 (C,F,I) wing buds was determined by whole-mount in situ hybridization. (J-N) Transverse vibratome sections of wing buds expressing Wnt5a (J-M) and Wnt11 and MyoD (N) at stages 22 (J,K), 26 (N) and 27 (L,M). In (A-M), expression is shown in purple/red. In (N), MyoD expression is shown in red and Wnt11 expression is shown in purple. In (A-N), anterior is uppermost and, in (A-I), distal is to the right. In the vibratome sections, (J) is more distal than (K), and (L) is more distal than (M). In (M), developing cartilage is circled in red, whereas developing muscle is circled in black. DM, dorsal muscle mass; R, radius; U, ulna; VM, ventral muscle mass. Scale bars: 100 µm in A,D,G; 150 µm in B,C,E,F,H,I; 125 µm in J,K; 150 µm in L,M.