Fig. 8. The effect of overexpression of activated components and dominant-negative components of the Wnt signalling pathway on fibre-type differentiation in vitro. The bar chart shows the total number of differentiated myogenic cells and the number expressing fast or slow MyHCs in 3-day limb micromass cultures that have been infected with either a control RCAS(BP) virus or retroviruses expressing DshPDZ, Lef, ß-catenin or activated CamKII. The slow population of myoblasts (red) might express either exclusively slow MyHC or both slow and fast MyHCs, whereas the fast myogenic population indicated by the yellow bar only expresses fast MyHC. *, P<0.05; **, P<0.01; ***, P<0.001.