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Fig. 2. Gdnf/Nt3 double null mutant mice do not differ from wild-type mice in LC cell numbers, expression of phenotypic markers or target innervation. The LC of wild-type (A,E-L), Gdnf null mutant (B), Nt3 null mutant (C) and Gdnf/Nt3 double null mutant (D,E'-L') were analyzed at P0. The number of LC NA neurons, assessed by in situ hybridization with riboprobes against DBH mRNA in wild-type, Gdnf

-/-, Nt3-/- and Gdnf/Nt3 double null mutants, did not differ (A-D). Moreover, immunohistochemistry for TH (E,E') and NOS (F,F') and in situ hybridization mRNA detection of BDNF (G,G'), NPY (H,H'), Galanin (I,I') and CGRP (J,J') did not differ in wild-type and Gdnf/Nt3 double null mutant mice. Finally, retrograde DiI tracing from the spinal cord (K,K') and nucleus accumbens (L,L') showed that the innervation of target structures in double null mutant mice does not differ from that in the wild-type mice.