Fig. 5. BDNF or NT4 increases the number of LCr NA neurons after 6 days in vitro. Dissociated rat E13.5 LCr primary cultures were treated as indicated on the x-axis. (A) Cell counts showed a tenfold increase in number of TH-positive cells for all conditions in which a TrkB ligand is present (*P<0.001, unpaired t-test). B-D show representative fields of LCr cultures with TH-positive cells after treatment with control media (B, N2) or N2 with BDNF (C) or NT4 (D). Scale bar: 100 µm.