Fig. 8. BDNF or NT4 selectively increased the number of Phox2a-positive cells that co-express TH. (A) BDNF increased the number of TH and Phox2a double positive cells between 3 and 6 hours in culture. This effect was maintained for up to 6 days in vitro (B). (B) Administration of either BDNF or NT4 selectively increased the pool of Phox2a-positive/TH-positive cells after 6 days in vitro, leaving the pool of Phox2a-positive/TH-negative cells unaffected. *P<0.05, unpaired t-test.