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Fig. 3. POP-1 in isolated AB descendants. (A,B) AB8 descendants of an isolated AB cell. (C,D) AB16 descendants of an isolated AB cell; the cell cluster has been flattened to visualize the majority of nuclei. (E,F) AB16 daughters of a sequentially isolated AB8 cell. (G,H) AB32 granddaughters of a sequentially isolated AB8 cell. POP-1 staining (left), corresponding DAPI-stained images (right); images of PKL-2 staining are superimposed on B,F and H with midbodies indicated by small arrows. Scale bars: 5 µm.