Fig. 4. Pvr protein is present on midline glia and hemocytes. (A-D) Wild-type stage 16 embryo stained with anti-Pvr (red) and anti-Peroxidasin (Pxn) (green). Yellow indicates overlap in the merged images (C,D). Pvr protein can be detected at the midline (arrowheads), in hemocytes (arrows) and at the surface of the embryo. (D) Higher magnification image of embryo depicted in A-C. The expression of Pvr in hemocytes is confirmed by co-expression with Peroxidasin in C and D. (E) Stage 17 embryo double-labeled with anti-Pvr (black) and BP102 (brown). Pvr protein is expressed on midline glia that surround the CNS commissures (arrowhead). (F) Higher magnification image of wild-type stage 16 embryo stained with anti-Pvr. CNS axon tracts are visualized using DIC optics. Pvr protein is expressed on midline glia (arrowheads) that surround the anterior and posterior CNS commissures. ac, anterior commissure. pc, posterior commissure. lo, longitudinal axons tracts. (G) Pvr^KO2 mutant embryo stained with anti-Pvr. Anti-Pvr staining cannot be detected in mutant animals.