Fig. 6. Macrophage function is required for proper CNS patterning. (A-D) Stage 16 (A) wild type, (B) Pvr^KO2,, (C) Srp^neo45 and (D) Crq RNAi embryos stained with BP102 to visualize CNS axons. Srp^neo45 and Crq RNAi embryos resemble Pvr embryos, with the axon scaffold having a rounded appearance in each segment (arrows). (E-H) Stage 16 (E) wild type, (F) Pvr^KO2,, (G) Srp^neo45 and (H) Crq RNAi embryos stained with Repo to visualize glia. Pvr^KO2, Srp^neo45 and Crq RNAi embryos all have disruptions in the pattern of Repo-positive glia. Srp^neo45 mutant embryos have increased numbers of Repo-positive glia at the midline in each segment (arrowheads). In Crq RNAi embryos, there are also increased numbers of glia at the midline (arrowheads), while the glia associated with longitudinal tracts appear more dispersed than in wild type (arrow).