Fig. 8. vri overexpression induces apoptosis and inhibits cell growth in larval tissues. Tissues were dissected at third larval instar. (A,B) Wing imaginal discs stained with Acridine Orange. (A) MS1096-GAL4/+ control disc with low level of apoptosis. (B) MS1096-GAL4/+; MS1096-GAL4/+; UAS-vri1⁹/+ with increased number of apoptotic cells (arrow) in the wing pouch region. (C,D) DAPI staining of male salivary gland. (C) F4-GAL4/+ control gland. (D) F4-GAL4/+; UAS-vri1⁹/+ gland (same magnification as control) with a size reduced by about one half and with pyknotic nuclei (arrows). (E,F) Fat body clones co-expressing vri and GFP induced by the `flip out' technique in hsp FLP; UAS-vri1⁹/Act5C<CD2<GAL4, UAS-GFP females. (E) GFP detection (green) with vri overexpressing clones of greater green intensity. (F) GFP and DAPI (red) stainings showing clones (yellow) with smaller cells.