Fig. 1. The coelomic blood vessel forms in the Wnt4^-/- ovary. (A) Light microscope images and Pecam stained images of kidney (kid), adrenal (ad) and ovary (ov) from 15.5 dpc embryos that were wild-type (+/+) and homozygous (-/-) for the mutant Wnt4 allele as indicated. (B) Gonads from 12.5 dpc embryos that were wild-type (+/+) or homozygous (-/-) for the mutant Wnt4 allele stained with a Pecam antibody, which labels the endothelium and germ cells at 12.5 dpc (speckled pattern in XX +/+ sample). In all images anterior is towards the left. Coelomic blood vessels are indicated by arrows and the ectopic vessel in Wnt4^-/- adrenals is indicated by an arrowhead.