Fig. 1. skd- or kto-mutant clones cross the AP compartment boundary. (A-H) Third instar wing discs containing mutant clones, with wild-type tissue labeled with GFP (green; C,D,G,H). Ci, marking anterior cells, is stained red (A,D,E,H). (A-D) skd^T606 clone. en-lacZ expression, detected by anti-ß-gal staining (blue), marks the posterior compartment. (E-H) kto^T241 clone. Anti-ß-gal staining (blue) revealing ptc-lacZ expression (F,H). Arrows indicate anterior clones that have crossed into the posterior compartment. (I,J) kto^T663 clone expressing HACi(m1-4), a form of Ci with all the PKA sites mutated. Anti-ß-gal staining (red) reflecting hh-lacZ expression; the clone is positively marked with GFP (J). Ci activation does not rescue the boundary crossing behavior. The compartment boundary is indicated by a white dashed line (D,H,J). (K) skd- and kto-mutant clones are rounder than their wild-type twin spots, and skd kto double-mutant clones are equally round. Circularity is measured as 4A/L², where A is the area of the clone and L is the perimeter, and would be 1.0 for a perfect circle. The mean for wild-type twin spots is 0.41, for skd clones is 0.67, for kto clones is 0.65 and for skd kto clones is 0.74. Lines within the bars indicate±1 s.d. P<0.001 for a comparison of skd, kto or skd kto to wild type. The differences between skd, kto and skd kto are not significant.