Fig. 2. MAP kinase-Gal4/Vp16 fusion constructs and their activity in the developing eye. (A) MAP kinase-Gal4/Vp16 constructs. Protein linear maps drawn to scale. NLS, SV40 nuclear localization sequence; MAPK, entire 286 residue natural sequence of the Drosophila rolled MAP kinase gene (Biggs and Zipursky, 1992); GAL4, residues 2-147 of the S. cerevisiae Gal4 (includes DNA-binding domain, but no known NLS sequence); VP16, residues 413-490 of HSV protein 16 (Sadowski et al., 1988); T, 11 residue epitope tag from HSV glycoprotein D (Novagen). (B-E) Eye discs are shown; anterior rightwards, dorsal upwards. In B-E, the fusion construct carried is indicated in the bottom left-hand corner and in all cases activity is detected antigenically using UAS:lacZ. Arrowheads indicate the furrow.