Fig. 8. Expression of MAPK directed to the nucleus affects development in the morphogenetic furrow. Eye discs are shown; anterior rightwards, dorsal upwards. A-D are at the same magnification; E,F are at the same magnification; G,H are at the same magnification. (AD) Atonal (green), Elav (red). (E,F) F-actin expression. (G,H) Elav expression. Note that at the end of the induction period (t=0) in HS:NM (C), Atonal expression is markedly reduced in the furrow (arrow in C), compared with wild type (A) or HS:M (B). Note that in HS:NM (D), 1 hour later Atonal expression has recovered. In addition, in HS:NM at t=0 the tight apical localization of F-actin markedly reduced in the furrow (arrow in F), compared with wild type (E, arrow). Elav expression appears precociously in the proto-R8 cells (arrowheads in H) compared with its normal first appearance in the proto-R2 and R5 cells (arrowheads in G).