Fig. 1. Movement of TVCVMP:GFP, 2xGFP and NLS:2xGFP. Confocal images of GFP fluorescence in inflorescence apices (A-C) and leaf epidermis (D-F) of 2-week-old ML1::TVCVMP:GFP (A,D), ML1::2xGFP (B,E) and ML1::NLS:2xGFP (C,F) transgenic plants. Inset in A shows that TVCVMP:GFP RNA is restricted to the L1, as detected by in situ hybridization. In D-F, the GFP fluorescence channel is overlaid with the transmissible light channel. TVCVMP:GFP is found in all cells in the inflorescence apex (A), and it is associated with the cell wall in a punctate pattern (D). 2xGFP forms a gradient of six to ten cells in the apex, with the highest concentration in L1 (B). It is located in both the nucleus and the cytoplasm (E). NLS:2xGFP can move efficiently only one cell layer from the L1 in the apex (C), and it appears mostly nuclear (F).