Fig. 2. Absence of movement of a functional AP1:GFP fusion. The C-terminal AP1:GFP fusion can rescue ap1-15 mutant flowers to near wild type when expressed from the AP1 promoter (A), and leads to the development of bracts subtending flowers when expressed in the L1 of wild type (C). The N-terminal fusion GFP:AP1 does not rescue ap1-15 mutant flowers when expressed from the AP1 promoter (B). Confocal images of GFP fluorescence in inflorescence apices of 2-week-old ML1::AP1:GFP (D,E) and ML1::GFP:AP1 (F) plants are shown. AP1:GFP does not move from the L1 (D; optical section through the shoot apex), and is tightly associated with nuclei (E; tangential section through the L1 of a floral primordium). By contrast, GFP:AP1 is largely cytoplasmic and moves into deeper tissues layers from the L1 (F).