Fig. 4. Western blots of whole-cell extracts from 2-week-old transgenic seedlings of ML1::2xGFP and ML1::NLS:2xGFP (A), ML1::AP1:GFP and ML1::GFP:AP1 (B), and ML1::GLFY, ML1:LFY:GFP and ML1::GFP:LFY (C) probed with anti-GFP antibody. Extract of wild-type Col-0 seedlings was used as a negative control in A. Blots were deliberately overexposed to reveal the presence of any minor bands. No major degradation products in the form of single GFP were found. Bands detected at higher molecular weight in the 2xGFP and GLFY lanes probably represent dimers formed during the extraction procedure. In B, GFP:AP1 bands are marked with an arrow and the minor single GFP band in GFP:AP1 is marked with an arrowhead.