Fig. 7. Movement of truncated LFY from the L1. (A) Diagram of GFP:LFY and the three truncated forms of the GFP:LFY fusion protein. GFP-coding sequence is shown in green and LFY-coding sequence in light blue, along with restriction sites used to make the deletions: B, BamHI; P, PstI; X, XbaI; S, StyI; H, HindIII. (B-D) Confocal images of GFP fluorescence in inflorescence apices of 2-week-old plants. All three forms of truncated GFP:LFY move from the L1 to the inner tissue layers. GFP:LFY1 is mostly located in the nucleus (B), whereas GFP:LFY2 appears to be largely cytoplasmic (C). Both can move several cell layers from the L1. GFP:LFY3 is mainly found in the form of large aggregates, but can still move one to two cell layers from the L1 (D).