Fig. 9. (A-C) The neural tube of embryos stained with Sytox (green) and the anti-aPKC antibody (red). Mitotic cells (arrows) in metaphase (A), anaphase (B) and telophase (C) are shown. Note that the epitope is expressed at the apical membrane of the mitotic cells. (D-F) A sequence of three time-lapse frames of a mitotic cell in the neural tube of an embryo injected with asip:egfp mRNA. Note that ASIP:EGFP (arrow in D) is located at the apical pole throughout mitosis (two arrows in F). (G-J) A sequence of four time-lapse frames of a mitotic cell in the neural keel of an embryo injected with asip:egfp mRNA. ASIP:EGFP is located at the apical pole of the neural keel cells (two black arrows in G), including the mitotic cell (white arrow in A). The apical localisation of ASIP:EGFP is lost when the cell enters mitosis, and in metaphase labelling is present in the entire cell membrane (four white arrows in H). After telophase (I), labelling reappears at the apical pole in the daughter cells (arrows, J).