Fig. 6. zag-1 expression pattern. DIC (A) and fluorescence (B) image of
zag-1::ZAG-1::GFP embryo with GFP expression in nerve ring nuclei
(int., intestinal autofluorescence). (C) zag-1::gfp(zdIs21) (2.9 kb
upstream fragment) adult hermaphrodite with expression in subset of nerve ring
neurons, no expression in ventral nerve cord neurons. (D) Tail region of
zag-1::gfp(zdIs21); zag-1(-918A,-895T)::rfp(zdIs41) adult.
zag-1 promoter (4.0 kb) containing mutated CACCT sites directed RFP
expression in more neurons than the wild-type promoter (GFP). Both promoters
directed expression in anal depressor and intestinal muscles. (E)
zag-1(zd86) zag-1::gfp(zdIs21). zag-1 mutations induced expression in
ventral cord motoneurons. (F) zag-1(-918A,-895T)::rfp(zdIs41). zag-1
promoter with mutated CACCT sites directed RFP expression in ventral cord
motoneurons. Anterior is leftwards (C), anterior is rightwards (D-F), dorsal
is towards the top.