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Fig. 1. Cloning of zag-1. (A) Genomic region containing the zag-1 gene. The fraction of transgenic lines rescuing zag-1 defects is indicated with numbers beside the cosmid names (3/4 – defects were rescued in 3 out of 4 transgenic lines). `Cosmid pool' refers to a mixture of all the cosmids shown. (B) ZAG-1 protein sequence. Zn fingers are indicated in bold, the homeodomain is underlined, the CtBP1/2 corepressor binding site (PLDLT) is in bold italics and the star indicates the premature stop codon in rh315. (C) Sequence alignments of Zn fingers. The sequence of the Zn fingers in the chick {delta}EF1 protein is identical to human ZEB. (D) Domain organisation of ZAG-1 and its homologs. Zn fingers are indicated in black, homeodomains in dark grey, SMAD-interacting domain in light grey and CtBP1/2 corepressor binding sites are dashed vertical lines.