Fig. 2. Expression of zag-1. zag-1::YFP: DIC (A-C) and corresponding epifluorescence images (D-F) of embryos at different developmental stages. zag-1 expression is mainly seen in neurons in the head and tail ganglia as well as in ventral cord motorneurons. Stars (F) mark two pharyngeal nuclei, probably m4 and m5 muscle cell nuclei. (G-H) Early (G) and late (H) L1 larvae; expression is seen transiently in postembryonic motorneurons (H). Arrowheads (H) indicate motorneuron nuclei in the ventral cord. Pzag-1::YFP in wildtype (I) and zag-1(rh315) animals (J-L); zag-1 expression in neurons is not downregulated in zag-1(rh315). Arrows (I,J,L) indicate intestinal muscle, arrowheads (I,J,L) indicate the anal depressor muscle. Ventral aspects in (I,J), side views in all others. Anterior is to the left, dorsal is up. Scale bars: 20 µm.