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Fig. 3. Neuronal defects in zag-1(rh315). Wild-type (A,C,E,G,I,K) and zag-1(rh315)-mutant animals (B,D,F,H,J,L) expressing glr-1::GFP (A-F), unc-47::GFP (G-J) and unc-4::GFP (K,L). zag-1(rh315) mutants have several axon outgrowth defects and misexpression of neuronal markers. Arrowheads (B,F) indicate cells ectopically expressing glr-1::GFP. Arrows (B) indicate lateral axons, (F) the PDB axon, which has a characteristic and unique trajectory, (H) a missing anterior commissure branch, and (J) a commissure growing on the wrong side. Ventral aspects in (C,D,I,J), side views in all others. Anterior is to the left, dorsal is up. Scale bars: 20 µm.