Fig. 5. Cloning of the FZP gene. (A) A map-based strategy was initially applied to clone the FZP gene but was discontinued with the identification of a transposon-tagged mutant. (Top) The markers flanking the FZP locus and the number of recombinants in a mapping population of 178 plants are indicated. FZP was delimited to a region of 119 kb covered by two PAC clones. (Bottom) The fzp-4 allele showed two Ac transposon insertions in a region corresponding to the AP004570 PAC clone; the distal Ac element disrupted a putative ERF-like gene. (B) Co-segregation of two Ac elements with the fzp phenotype (arrowheads) in fzp-4 seedlings. (Left) Southern hybridization of four seedlings of the parental Ac 26-6 line. (Right) Southern hybridization of four seedlings of the progeny of the Ac 26-6-1 seedling. The genotype (G) of the fzp locus disruption and phenotype (P) of each seedling are indicated below. +/-, heterozygous; -/-, homozygous mutant; +/+, homozygous wild type; wt, wild-type; fzp, frizzy panicle. M, marker: 3, 5, 6, 8, 10 kb from the bottom to the top.