Fig. 2. fue zygotes resume and complete meiosis normally. DAPI was applied in order to label chromosomes (blue) and fluorescein phalloidin to visualise the actin cytoskeleton (green). (A,B) Chromosomes during meiotic arrest at metaphase II in wild-type (A) and fue (B) unfertilized, non-activated oocytes. Directly after fertilization meiosis is resumed and completed with the extrusion of one set of chromosomes into a vesicle on the outside of the cell membrane. Extrusion of this second polar body (arrowhead) takes place around 7 minutes post-fertilization in wild-type (C) and fue zygotes (D). This demonstrates that the terminal event of meiosis is completed in fue zygotes as in the wild type. Scale bars: 10 µm.