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Fig. 5. Eya1, Pax2 and Pax8 expression in otic epithelium is Six1-independent. (A,B) Six1lacZ is normally expressed in otic vesicle (ov) and its expression was undetectable in Eya1-/- embryos. (C-H) Eya1 (C,D), Pax2 (E,F) and Pax8 (G,H) expression levels in otic vesicle were unaffected in Six1-/- embryos. Scale bars: 100 µm. nt, neural tube.