Fig. 6. The N-terminal region and the SH2 domain of tensin are required for normal wing development. (A) Various regions of by subcloned into pUAST vector were overexpressed in Drosophila as described in the Materials and Methods. (B) Determination of expression levels of by fragments in overexpression lines using RTPCR method. `a' and `b' represent 5' and 3' regions of the by gene, respectively (shown in A). Ribosomal protein 49 (Rp49) was used as an internal control. FL, overexpression of full-length by. All RT products were amplified 25 cycles in PCR reactions. (C) MS1096/X; UAS-PTB/+; by²/by². (D) MS1096/X; by²,UAS-N/by². (E) MS1096/X; UAS-C/+; by²/by².