Fig. 9. Induction of apoptosis and JNK activity by by. (A-C) In immunohistochemical analyses using anti-phosphospecific JNK antibody, JNK phosphorylation was dramatically increased in the wing imaginal discs expressing by (C), and decreased in the wing imaginal discs of homozygous by² mutant (B) compared with the wild-type control (A). (D) The MS1096-GAL4 fly displayed a normal wing phenotype. (E) Overexpression of by using MS1096-GAL4 turned the wing into a convex shape. (F,G) In Acridine Orange staining experiments, overexpression of by (G) dramatically induced apoptotic cell death in wing imaginal discs, compared with the wild-type control (F).