Fig. 2. Defective stomata are binucleate. Cotyledons of 13-day-old wild-type (A) and scd1-1 seedlings (B,C) that express the nuclear (green) GFP N7 marker and (D) cotyledons from 4-day-old seedlings expressing plasma membrane GFP 29-1 marker (Cutler et al., 2000). Multinucleate scd1-1 epidermal cells with wall stubs (arrow) are shown in C and D. Two serial confocal optical sections were merged to create C. Cell walls were stained with propidium iodide (red) and chloroplasts are autofluorescent (blue). (D) The membrane in the cell wall stubs of cytokinesis-defective scd1-1 epidermal cells was contiguous with the plasma membrane. Scale bars: 10 µm.