Fig. 4. The scd1-1 mutant is temperature-sensitive. (A) scd1-1 plants grown under continuous illumination at 22^oC (left) and 16°C (right). (B) Aborted scd1-1 flower buds from plants grown at 22°C. (C) The fertile inflorescences of plants grown at 16°C. (D,E) Toluidine Blue stained leaf epidermal peels from plants grown at 22°C (D) show many defective guard cells whereas plants grown at 16°C (E) show normal guard cells and an increased number of meristemoids. (F) Temperature-shift experiment. scd1-1 flower buds that were developing during the 16°C incubation period (bracket) developed into fertile flowers, which produced siliques filled with scd1-1 seed. Upon return to restrictive conditions (22°C), flower bud development aborted once again. (G) Three-month-old scd1-3 mutant grown in soil at 18°C. Scale bars: 1 cm (A); 0.5 cm (B,C); 10 µm (D,E); 0.25 cm (G).