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Fig. 6. HLH-2 is most probably present in the NSM sister cells. (Upper panel) Anti-HLH-2 and anti-GFP immunofluorescence staining of a 1.5-fold embryo of genotype bcIs1 (Pegl-1gfp); ced-3(n717), and overlay (from left to right). The images presented are stacks of a confocal series through an entire embryo. The four double positive cells found in embryos of this genetic background are indicated by arrows. (Lower panel) Anti-HLH-2 and anti-GFP immunofluorescence staining of a 1.5-fold embryo of genotype unc-87(e1216) ces-1(n703gf); bcIs1 (Pegl-1gfp); ced-3(n717), and overlay. The images presented are stacks of a confocal series through an entire embryo. The two double positive cells found in embryos of this genetic background are indicated by arrows.