Fig. 7. CES-1 and an activator of egl-1 expression function through the Snail-binding sites/E-boxes in Region B in vivo. Plasmids pBC08 (wild-type Region B) (A), pBC181 (Region B with Snail^-/E-box^- sites) (B) or pBC182 (Region B with Snail^-/E-box⁺ sites) (C) were introduced by germline transformation into hermaphrodites of genotype ces-2(n732ts); bcIs25; egl-1(n1084 n3082) unc-76(e911) as described in the Materials and Methods. Transgenic lines were cultured at 15°C and 25°C. Rescue of the NSM sister cell death was analysed as described in the Materials and Methods (n=60-222). For every plasmid, several independent transgenic lines were generated and analysed (n=3-6).