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Fig. 6. Overexpression of AS2 suppresses KNAT1 expression. (A-F) Same-stage inflorescences. (A) Ler, (B) as1-101, (C) 35S::AS1/Ler, (D) as2-101, (E) 35S::AS2/Ler, (F) bp. Note that 35S::AS2 transgenic plants and bp mutant plants exhibit similar altered inflorescence architecture, with downward-pointing siliques. (G) RT-PCR. RNA was extracted from the primary inflorescence, and the amplified DNA fragments were separated by electrophoresis on an agarose gel and visualized by staining with ethidium bromide. Scale bars: 1 mm (A-F).