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Fig. 8. AS1 and AS2 interaction in yeast and in ELISA. (A,B) Yeast two-hybrid analyses by coexpression of AS1 and AS2, showing that the interaction of AS1 and AS2 turns on the reporter genes lacZ (A) and HIS3 and ADE2 activities (B). (C) Recombinant purified proteins His-AS1 and GSTAS2 were analyzed by SDS-PAGE and stained by Coomassie Blue R-250. Note, sizes of the recombinant His-AS1 and GST-AS2 proteins agreed with their predicted molecular masses. M, molecular mass marker (in kDa); GST, glutathione S-transferase control; GST-AS2, GST tag and AS2 fusion protein; His-AS1, His tag and AS1 fusion protein. (D) ELISA was performed showing protein-protein interaction between the His-AS1 and GST-AS2.