Fig. 2. Comparison of the wing region of a wild-type and dve^P1738-mutant wing imaginal disc, in animals of the late third larval instar stage and of the early pupal stage. The discs are double stained with anti-ß-Gal (green) and anti-Wg (red) antibodies. (A) A wild-type wing imaginal disc of the late third larval instar stage. (C) A dve-mutant wing imaginal disc of the late third larval instar stage. Anterior is to the left; ventral to the bottom. The arrow (A,C) indicates the wg-expression domain along the DV boundary. ir, the inner ring-like expression domain of wg in the PW. The comparison of A and C reveals that the wing pouch of the dve-mutant wing disc is smaller. As a consequence the distance between the DV boundary and the inner ring-like domain of wg expression is reduced. (B,D) The defects become more obvious in wing imaginal discs of the early pupal stage. The dve-mutant wing (D) is smaller than its wild-type counterpart (B). The arrow highlights an indentation in the anterior margin of the mutant wing. Furthermore, the proximal fold, which is labelled by the asterisk (B,D) is smaller in the dve mutant (compare the distance between the two arrowheads in B and D).