Fig. 7. Comparison of retinal cell distribution in tadpoles carrying and lacking the Xrx1A-FGFR4a construct. (A) Immunostaining of an eye section from a stage 45 non-transgenic tadpole with antibodies against Islet1, which recognizes the ganglion and amacrine cells. (B) Immunostaining of an eye section from a stage 45 tadpole that carries the Xrx1A-FGFR-4a construct with antibodies against Islet1, demonstrating disturbed layering of retinal cells. (C) Hoechst staining of the section from B. (D) Immunostaining of an eye section from a stage 45 tadpole that does not carry the Xrx1A-FGFR4a construct with antibodies against glutamine synthetase, which recognizes Müller cells. (E) Immunostaining of an eye section from a stage 45 tadpole that carries the Xrx1A-FGFR4a construct with antibodies against glutamine synthetase demonstrates irregular distribution of Müller cells in the retina of these tadpoles. (F) Hoechst staining of the section from E. (G) Histogram showing the percentage of Müller glial cells and retinal ganglion cells/amacrine cells in the retina of transgenic tadpoles. Müller glial cells and retinal ganglion cells/amacrine cells are identified by immunostaining with antibodies against glutamine synthetase and Islet1, respectively. MGC, Müller glial cells; RGC, retinal ganglion cells; AC, amacrine cells; Single Tsg, car-GFP transgenic (MGC, n=8 retinas; RGC/AC, n=6 retinas); Double Tsg, car-GFP/Xrx1A-FGFR4a transgenic (MGC, n=10 retinas; RGC/AC, n=11 retinas).