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Fig. 5. Transient expression assays of the 2.2 kb upstream sequence injected into L. variegatus eggs. (A) Microinjection of a LvEndo16-GFP reporter construct resulted in fluorescence in the vegetal plate at the mesenchyme blastula stage. (B) During gastrulation, fluorescence is detected in the archenteron. (C) A ventral view showing fluorescence in the midgut of the pluteus larva. (D) A lateral view showing fluorescence in both the midgut and hindgut of the pluteus larva.