Fig. 4. GFP-Bry expression relative to Flk1, CD31 and Kit. (A) At the indicated time points, GFP-Bry EBs were harvested and dissociated. The cells were stained with Flk1, c-Kit or CD31 mAb and analyzed by flow cytometry. (B) The level of Kit marker was assessed for each subpopulation delineated by Flk1 and GFP-Bry expression at day 3 of differentiation. The mean fluorescence intensity (MFI) is given for each graph. At day 0 of differentiation, the ES cells expressed Kit. The broken line in this graph represents the unstained control.