Fig. 6. Protein localisation in lines expressing an ACR4::GFP protein fusion. (A) localisation of fusion proteins (gren) in the plasma membranes of the ovule epidermis. (B,C) Expression patterns of fusion proteins (green) in embryos (B) and meristems (floral meristem, fm) (C). Plasma membrane localisation can be observed (arrow in B). (D-F) Protein localisation in root meristems. (D) Lateral root tip, columella and LRC expression are visible. No protein is seen in the epidermis (e) before emergence from the LRC. (E) Main root tip with columella cell layers indicated by stars. (F) surface view when mounted in 0.8 M mannitol. Cell wall area is clear of fluorescence. (G,H) Protein localisation in comparable untreated and 2-hour BFA-treated root samples, respectively, showing relative decrease in plasma membrane localisation and the appearance of bright perinuclear bodies. (I) mature ovule with fluorescence seen in outer integument (oi) (where little protein is detected in the outermost cell plasma membrane; arrowhead), inner integument (ii) and outer cell layer of funiculus (f). rp, embryonic root pole; c, cotyledon primordia; s, sepal primordia; m, micropyle; fg, female gametophyte. Scale bars: 25 µm.