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Fig. 7. Wise blocks cell movements in activin-treated animal caps. (A) Control animal caps at stage 15. (B) Control animal caps treated with activin (8 units/ml). They undergo gastrulation-like movements in response to activin and become elongated. (C) Animal caps from Wise RNA (1 ng) injected embryos, treated with activin. Elongation is blocked. (D) RT-PCR of animal caps assayed for muscle-actin (m.a.) and ef1{alpha}. All caps were assayed at stage 15. Induction of muscle actin by activin is not interfered by preceded injection of 500 pg (+) or 1 ng (++) of Wise RNA, showing that failure in elongation in response to activin in C is not due to failure in muscle induction.