Fig. 3. Comparison of endogenous her5 (blue) and gfp (red) RNA transcription profiles in her5PAC:egfp (A,C,D) and -3.4her5:egfp (B,E,F) transgenic embryos, at the stages indicated. All views are high magnifications of the MH area in flat-mounted embryos, dorsal (A,B,E,F and inset in D) or sagittal (C,D) orientations, anterior towards the top (A,B) or left (C-F). Endogenous her5 and gfp expressions exactly coincide at all embryonic stages, including the initiation (A,B) and maintenance (C-F) phases of her5 transcription, demonstrating that all the regulatory elements driving MH her5 expression are contained within the her5PAC:egfp and -3.4her5:egfp constructs.