Fig. 1. Tissue-specific expression of a GFP
KN1 fusion produces transgenic
plants carrying distinct phenotypes. (A) Plants expressing mGFP5-ER in the
mesophyll had a normal phenotype. (B,C) Epidermis-specific expression of
GFP
KN1 using pLTP1 (B) or pAtML1 (C) resulted a mildly
rumpled leaf phenotype. (E) Mesophyll-specific GFP
KN1 expression resulted
in lobed leaf phenotypes (D) that were reminiscent of plants overexpressing
KNOX genes using the 35S promoter. (E) Representative leaves of
plants shown in A-D are displayed in order. (F,G) Severe
pRbcS2b-GFP
KN1 seedlings: (F) SEM and (G) a confocal image
showing an ectopic shoot on the adaxial side of the leaf (G). Scale bars: 1 cm
(A-E), 1 mm (F), 50 µm (G).