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Fig. 4. Tc-ASH is necessary and sufficient for neural precursor formation. (A-C) Tribolium embryos injected with (A) buffer or (B, C) Tc-ASH double stranded RNA stained for Tc-ase RNA (blue) and Engrailed protein (brown). (A) Buffer-injected embryo displays the wild-type pattern of neural precursors. (B,C) Tc-ASH RNAi-treated embryos display either (B) a complete or (C) a partial loss of neural precursors as judged by Tc-ase expression. (D-F) Dorsal view of adult Drosophila nota. (D) Wild-type pattern of sensory bristles. (E,F) Ectopic sensory bristles result from misexpression of either (E) UAS-Drosophila sc or (F) UAS-Tc-ASH under the control of the apterous-Gal4 driver line. A-C: anterior, left; D-F: anterior, up.